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The virus is harvested from infected human diploid cells, MRC-5 strain, concentrated by ultrafiltration and is inactivated by beta propiolactone. This vaccine must only be used intramuscularly as a single dose. The potency of one dose (1.0 ml) of Aventis Imovax rabies vaccine is equal to or greater than 2.5 international units of rabies antigen.

Seroconversion was often obtained with only one dose. With 2 doses one month apart, 100% of the recipients developed specific antibody and the geometric mean titer of the group was about 10 international units.