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polyionic complexes have a solubility problem, due to the neutralization of
charges. In order to resolve this problem this type of compound is joined to
a nonionic polymer soluble in water: polyethyleneglycol (PEG), resulting in
an increase in the solubility, a decrease in the toxicity and an increasein
the efficiency of transfection.
PEI favors high levels of expression of a transgene after administration by aerosol, with toxicity and cytokine response minimum and without acute inflammation. Via this type of system, antitumeral effects have been attained in 2 different cancer models using the suppresser gene p53.
An aerosol system has been used in the murine melanoma B16-F10 for the administration of the PEI-p53 complex, achieving significant tumor reduction and a 50 % increace in survival. The mechanism of these p53 antitumoral effects is due in part to the supression of angiogenesis, due to the overregulation of the angiogenic factor thrombospondine 1 (TSP-1) in the lung tissue and under low regulation of the vascular endothelium factor (VEGF).