|front |1 |2 |3 |4 |5 |6 |7 |8 |9 |10 |11 |12 |13 |14 |15 |16 |17 |18 |19 |20 |21 |22 |23 |review|
as previously described, other methods are used in this type of therapy such
as the use of antisense oligonucleotides that block the transduction of mRNA
to protein and ribozymes that reduce the average life of mRNA. Lung cancer
cells have been treated in vivo and in vitro with antisense segments for
cycline D1, reducing the cellular proliferation and the tumorigenicity.
The oncogene HER-2/neu codes for a transnmembranal protein with tyrosine kinase activity and overexpression in many types of cancers, correlating with a bad prognosis and chemoresistance in lung cancer.
The product of the early gene 1 A of adenovirus type 5 (E!A) can inhibit the expression of HER-2/neu, and with this strategy inhibit the intratracheal growth of pulmonary cancerous cells in the murine model.
Another potential gene has been studied, the insulin growth factor IGR-Ir. In a murine model, it has been used like an adenovirus vector of the antisense sequence resulting in a longer life. Other genes include the epidermal growth factor receptor (EGFR) gene of chromosome (7p12), the C-MYC gene (8q24), chromosome 5 (5p15.2) and chromosome 6, which can be used to detect lung cancer when the number of copies is ± 2. This immunohistology is conducted by DNA fluorescence in situ hybridization (FISH).