A total of 5 1-mL doses is administered intramuscularly in the upper arm, one each on days 0, 3, 7, 14 and 28. Human rabies immune globulin (HRIG) is also administered, up to one week after the first dose of vaccine. As much as possible of the full dose of very costly HRIG should be injected into and around the site of exposure to block entry of the virus into the CNS. Any remaining HRIG should be injected intramuscularly at a site away from the vaccine.

Laboratory workers who have contact with the rabies virus or who are involved in rabies diagnosis, veterinarians, technical staff and students, animal control, wildlife and zoo workers, and spelunkers (people who explore caves) all should be vaccinated against rabies.