University of Pittsburgh

School of Pharmacy

 


Robert Gibbs, Ph.D.

Professor of Pharmaceutical Sciences

Director, Cell Imaging Core

University of Pittsburgh School of Pharmacy, 1004 Salk Hall, Pittsburgh, PA 15261

e-mail: gibbsr@pitt.edu


Research

Dr. Gibbs's laboratory is located on the 10th floor of Salk Hall where investigators are studying the ability of gonadal hormone therapy to enhance and maintain the functional status of specific neural systems in the adult and aging brain. Current studies are focusing on the effects of estradiol and testosterone on cholinergic projections to the hippocampus and cortex, the enhancement of which may help to prevent or delay the development of Alzheimer's-related dementia in postmenopausal women.

 


Training Opportunities

A wide variety of molecular, histochemical, and behavioral techniques are used in the laboratory to study how brain function is affected by gonadal hormones and aging. Some of the techniques currently being utilized include quantitative RT-PCR, quantitative in situ hybridization histochemistry, immunocytochemistry, in vivo microdialysis, enzyme assays, uptake assays, and measures of learning and memory. Students are encouraged to acquaint themselves with all of the techniques being used in the laboratory. Opportunities for additional predoctoral and postdoctoral training in the Department of Pharmaceutical Sciences and in conjunction with the Center for Reproductive Physiology are also available.


 

Recent Publications

 

Li, J., P. J. Oberly, S. Polloyac, R.B. Gibbs (2016) A microsomal based method to detect aromatase activity in different brain regions of the rat using ultra performance liquid chromatography-mass spectrometry. The Journal of steroid biochemistry and molecular biology. Journal of Steroid Biochemistry and Molecular Biology. 163:113-120.

 

Lu, J., W. Zhao, Y. Huang, H. Liu, R. Marquez, R.B. Gibbs, J. Li, R. Venkataramanan, L. Xu, and S. Li, (2014) Targeted delivery of Doxorubicin by folic acid-decorated dual functional nanocarrier. Molecular pharmaceutics. 11(11): p. 4164-78. PMCID 4224520

 

Gibbs, R.B., Nelson, D., Hammond, R.E. (2014) Role of GPR30 in mediating estradiol effects on acetylcholine release in the hippocampus, Hormones & Behavior, 66(2): 339-345. PMCID 4131743

 

Ihunnah, C.A., T. Wada, B.J. Philips, S.K. Ravuri, R.B. Gibbs, L. Kirisci, J.P. Rubin, K.G. Marra, and W. Xie. (2014) Estrogen sulfotransferase/SULT1E1 promotes human adipogenesis. Molecular and cellular biology, 34(9): p. 1682-94. PMCID 3993609

 

Ramaswamy, S., Dwarki, K., Ali, B., Gibbs, R.B., and Plant, T.M. (2012) The decline in pulsatile GnRH release, as reflected by circulating LH concentrations, during the infant-juvenile transition in the agonadal male rhesus monkey (Macaca mulatta) is associated with a reduction in kisspeptin content of KNDy neurons of the arcuate nucleus in the hypothalamus, Endocrinology, 154(5): p. 1845-53. PMCID 3628021

 

Babalola, P.A., Fitz, N.F., Gibbs, R.B., Flaherty, P., Li, P.-K., Johnson, D.A. (2012) The Effects of The Steroid Sulfatase Inhibitor (p-o-sulfamoyl)–Tetradecanoyl Tyramine (DU-14) on Learning and Memory in Rats with Selective Lesion of Septal-Hippocampal Cholinergic Tract, Neurobiol. Learning and Memory, 98(3): 303-310. PMCID: 3491137

 

Hammond, R., Nelson, D., Kline, E., and Gibbs, R.B. (2012) Chronic treatment with a GPR30 antagonist impairs acquisition of a spatial learning task in young female rats, Hormones & Behavior, 62(4):367-74. PMCID: 3477261

 

Zhang, Y., Huang, Y., Zhang, P., Gao, X., Gibbs, R.B., and Li, S. (2012) Incorporation of a selective sigma-2 receptor ligand enhances uptake of liposomes by multiple cancer cells, International Journal of Nanomedicine, In Press

 

Cai, L., Gibbs, R.B., Johnson, D.A. (2012) Recognition of novel objects and their location in rats with selective cholinergic lesions of the medial septum. Neuroscience Letters, 506(2): 261-265. PMID: 22119001

 

Gibbs, R.B., Chipman, A.M., Hammond, R. and Nelson, D. (2011) Galanthamine Plus Estradiol Treatment Enhances Cognitive Performance in Aged Ovariectomized Rats, Horm. & Behav.60:607-616. doi:10.1016/j.yhbeh.2011.08.010.PMID: 3210334

 

Gibbs, R.B., Chipman, A.M., and Nelson, D. (2011) Donepezil Plus Estradiol Treatment Enhances Learning and Delay-Dependent Memory Performance by Young Ovariectomized Rats with Partial Loss of Septal Cholinergic Neurons, Horm. & Behav., 59(4): 503-11. DOI S0018-506X(11)00026-2 [pii] 10.1016/j.yhbeh.2011.01.011; PMID 3081392

 

Hammond, R.*, and Gibbs, R.B. (2011) GPR30 is positioned to mediate estrogen effects on basal forebrain cholinergic neurons and cognitive performance, Brain Res, 1379:53-60, doi:10.1016/j.brainres.2010.11.098; PMID 3046317

 

Pokala, V.N., Witt-Enderby, P.A., Gibbs, R.B., and Johnson, D.A. (2010). Selective septohippocampal cholinergic lesioning in rat brain: Effects on muscarinic receptor function and secretase enzyme activity. Curr. Topics in Pharmacol. 14: 73-78.

 

Kline, A.E., McAloon, R.L., Henderson, K.A., Bansal, U.K., Ganti, B.M., Ahmed, R.H., Gibbs, R.B. and Sozda, C.N. (2010) Evaluation of a Combined Therapeutic Regimen of 8-OH-DPAT and Environmental Enrichment After Experimental Traumatic Brain Injury, J. Neurotrauma, 27:11, 2021-2032. DOI: 10.1089/neu.2010.1535; PMID 2978058

 

Hammond, R.*, Nelson, D., and Gibbs, R.B. (2010) GPR30 co-localizes with cholinergic neurons in the basal forebrain and enhances potassium-stimulated acetylcholine release in the hippocampus. Psychoneuroendocrinology, 36(2):182-192, DOI: 10.1016/j.psyceuen.2010.07.007. PMID: 2994977

 

Gibbs, R.B. (2010) Does Short-Term Estrogen Therapy Produce Lasting Benefits In Brain? Endocrinology, 15(3): 843-845.

 

Gibbs, R.B. (2010) Estrogen therapy and cognition: A review of the cholinergic hypothesis, Endocrine Reviews 31(2): 224-253. PMID: 2852210.

 

Hogervorst cover.pdf


Complete Publication List

Robert Gibbs - CV


Other sites of interest:

University of Pittsburgh School of Pharmacy

Masters and PhD Programs in Pharmaceutical Sciences

Center for Research in Reproductive Physiology

Alzheimer's Disease Research Center