BIOL 1401: Developmental Biology
Spring 2003
Instructor: Lauren Yaich, Ph.D.
Telephone: 362-0260
Office: 203 F Fisher Hall
E-mail: yaich@pitt.edu
Lectures: M W F 3 - 3:50 pm
Lab: Thurs. 2:30 - 6:30 pm
Office Hours: M W F 1 - 3 pm
Also by appointment or on a "drop-in" basis
Textbook: Wolpert, L., Beddington, R., Jessell, T., Lawrence,
P., Meyerowitz, E, and Smith,
J. Principles of Development, Second Edition. Oxford University
Press: Oxford, 2002.
*Additional readings will be placed on reserve in the library.
Lab Manual: Tyler, M. S. Developmental Biology: A Guide
for Experimental Study, Second Edition. Sinauer Associates:
Sunderland, MA, 2000.
Class Web-Sites: http://courseweb.pitt.edu
and http://www.pitt.edu/~yaich/classes.htm
Class Description and Philosophy:
In Developmental Biology, we will discuss the development of plants
and animals, with a particular emphasis on the molecular genetic
basis for developmental events. The entire span of development will
be examined, from the formation of germ cells and fertilization
through embryonic development and even up through post-embryonic
development, senescence, and death. The laboratory exercises for
this course will focus on developmental phenomena studied in several
of the most prominently utilized model organisms including Dictyostelium,
Caenorhabditis elegans, Drosophila melanogaster, Danio rerio, amphibians,
and chicks. Developmental Biology will also emphasize the reading
of primary literature, culminating in a poster session near the
end of the term. A basic understanding of genetics and cell biology
by the student is assumed. If you have not had Cell Biology and
Genetics, please come see me immediately to discuss whether you
have the proper prerequisites for this course.
______________________________________________________________________________
Lecture Schedule
January
6 Introduction to Developmental Biology - Chapter 1
8 History and Basic Concepts - Chapter 1
10 Vertebrate Model Systems - Chapter 2
13 Invertebrate and Plant Model Systems - Chapter 2
15 Patterning the Vertebrate Body Plan: Axes and Germ Layers (I)
- Chapter 3
17 Patterning the Vertebrate Body Plan: Axes and Germ Layers (II)
- Chapter 3
20 No Class - Martin Luther King, Jr. Day
22 Patterning the Vertebrate Body Plan: - Chapter 4
Mesoderm and Nervous System (I)
24 Patterning the Vertebrate Body Plan: - Chapter 4
Mesoderm and Nervous System (II)
27 Case Study #1: Defects of Vertebrate Pattern Formation - TBA
29 Discussion Session
31 Lecture Exam #1
February
3 Drosophila Development (I) - Chapter 5
5 Drosophila Development (II) - Chapter 5
7 Film: "Hopeful Monsters"
10 Development of Other Invertebrates (I) - Chapter 6
12 Development of Other Invertebrates (II) - Chapter 6
14 Plant Development (I) - Chapter 7
17 Plant Development (II) - Chapter 7
19 Case Study #2: Model Organisms and the Human Connection - TBA
21 Discussion Session
24 Lecture Exam #2
26 Morphogenesis (I) - Chapter 8
28 Morphogenesis (II) - Chapter 8
March
3 Spring Break! - No Class
5 Spring Break! - No Class
7 Spring Break! - No Class
10 Cell Differentiation (I) - Chapter 9
12 Cell Differentiation (II) - Chapter 9
14 Organogenesis (I) - Chapter 10
17 Organogenesis (II) - Chapter 10
19 Development of the Nervous System (I) - Chapter 11
21 Development of the Nervous System (II) - Chapter 11
24 Case Study #3: Holoprosencephaly and Signal Transduction -
TBA
26 Discussion Session
28 Lecture Exam #3
31 Germ Cells and Sex (I) - Chapter 12
April
2 Germ Cells and Sex (II) - Chapter 12
4 Regeneration - Chapter 13
7 Growth - Chapter 14
9 Post-Embryonic Development - Chapter 14
11 Evolution and Development (I) - Chapter 15
14 Evolution and Development (II) - Chapter 15
16 Case Study #4: Regeneration - Fooling Mother Nature - TBA
18 Discussion Session
Final Exam
Wednesday, April 23 3-5 pm
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Laboratories
The labs will consist of both descriptive and experimental exercises.
Attendance at laboratories is required. Any unexcused absence from
a lab session will result in a 10-point deduction from your lab
grade. Occasionally you may be asked to set up or take down part
of an experiment at other times than the standard lab period. In
addition to your lab manual, there will be handouts for most of
the laboratories. You will be expected to have done the appropriate
readings before coming to lab! The lab grade will consist of both
exercises and a lab practical exam at the end of the course. Therefore,
you should take the laboratories seriously and make an attempt to
understand clearly what you are doing in lab throughout the semester!
Make notes and observations in your laboratory manual and handouts
and hang on to them until the final lab exam.
Lab Schedule
(Tentative)
January
9 Introduction to the Developmental Biology Lab - Handout
16 Vertebrate Development I (Frog and Zebrafish) - Handout
23 Vertebrate Development II (Chick) - Chapters 9 & 10
30 UAS-GAL4 Overexpression and GFP Experiments: Part I - Handout
Immunohistochemistry: Part I - Handout
February
6 Drosophila Development - Chapter 8
Immunohistochemistry: Part II
13 UAS-GAL 4 Overexpression Handout
and GFP Experiments: Part II
Immunohistochemistry: Part III
20 C. elegans Development - Handout
Immunohistochemistry: Part IV
27 Dictyostelium Development - Chapter 4
March
6 No Lab - Spring Break !
13 Developmental Biology Databases - Handout (Meet in CALC Lab)
20 Teratogens and Development - Handout
27 Planaria Regeneration Experiment - Chapter 13
April
3 Poster Session
10 Gametogenesis - Chapter 5
17 Lab Exam
Lab Safety Policy:
Students are expected to demonstrate suitable laboratory conduct
and to practice standard laboratory safety procedures. Approved
safety glasses must be worn at all times when working with chemicals
or doing procedures which may involve potential eye hazard. It is
the student's responsibility to provide their own safety glasses
(and/or lab coat if desired). Safety glasses can be purchased in
the bookstore at a modest cost. Lab coats are available at most
uniform supply stores. While a lab coat is not required, it is recommended
that you do not wear "good" clothes to lab. If you must
do so, cover them up with a lab coat or an old shirt. No eating,
drinking, or smoking will be tolerated in the lab. Treat all chemicals,
biological materials, and lab equipment with respect. Failure to
do so can adversely affect one's experimental results or personal
well being. Remember to report all accidents to the instructor,
no matter how minor they seem to be. Additional safety rules specific
to the Developmental Biology Lab will be specified in the laboratory
handouts.
Grading
Lecture Exam #1 100 points
Lecture Exam #2 100 points
Lecture Exam #3 100 points
Final Exam 100 points
Case Studies 80 points
Poster 100 points
Lab Exercises 100 points
Lab Exam 100 points
Total Points = 780 points
Final Grades will be calculated as follows:
Total Number of Points Earned / 780 points X 100 = Final % Grade
The percentile grade will correspond to the following letter grades:
A+ = 96.7-100 %
A = 93.4-96.6 %
A- = 90.0-93.3 %
B+ = 86.7-89.9 %
B = 83.4-86.6 %
B- = 80.0-83.3 %
C+ = 76.7-79.9 %
C = 73.4-76.6 %
C- = 70.0-73.3 %
D+ = 66.7-69.9 %
D = 60.0-66.6%
F = <60 %
Class Policies:
1. Attendance at lectures is strongly recommended. Most (but not
necessarily all!) of the material appearing on the exams will be
based on lectures. Attendance at labs is REQUIRED. An unexcused
absence from lab will result in a 10-point deduction from the lab
grade. If you will be missing a lecture exam or lab for a legitimate
reason, contact me as soon as possible, preferably in advance. More
than two absences in a semester due to illness will necessitate
a note from your doctor or the college nurse. Make-up exams will
only be given if I feel that the absence was justifiable. Arrangements
for any make-ups should be made within three days of the absence.
2. A "poster" is a way of presenting scientific information
at meetings and conferences. In place of a term paper we will be
having a poster session in which each student will present a poster
detailing library research on a particular topic related to developmental
biology. This will be a significant part of your grade, so I recommend
beginning work on this early in the semester. I will pass out a
handout giving more information regarding this assignment.
3. I may occasionally offer an extra credit assignment at various
times throughout the semester. This is primarily to allow you to
catch up on a few points if you had bad luck on an exam or assignment.
However, these will only be worth a minimal number of points! Extra
credit alone will not be able to take you from a D to an A, so you
should try to do your best in all aspects of this class.
4. Please establish your personal e-mail account as soon as possible.
Any special announcements will be made by e-mail and/or in lecture.
Also, e-mail is a great way to reach me for simple questions or
to arrange for an appointment.
5. This syllabus is subject to change. While I will try my best
to adhere to the schedule, occasionally changes may be necessary.
If so, I will announce the change(s) both in lecture and on Blackboard.
6. Students in this class will be expected to adhere to The University
of Pittsburgh at Bradford's Academic Integrity Policy, which reads
as follows:
Members of a university community, both faculty and students,
bear a serious responsibility to uphold personal and professional
integrity and to maintain complete honesty in all academic work.
Violations of the code of academic integrity are not tolerated.
Students who cheat or plagiarize or who otherwise take improper
advantage of the work of others, face harsh penalties, including
permanent dismissal. The academic integrity guidelines set forth
student and faculty obligations and the means of enforcing regulations
and addressing grievances.
Please pay particular note to the prohibition against plagiarism.
You must use your own words in any written work! Any evidence of
plagiarism will immediately result in a failing grade for that assignment.
Continued plagiarism beyond this or cheating on exams will potentially
result in the student being brought before administrative review.
If you are unsure of how to properly "paraphrase" sources,
please come see me and I will be happy to help you with this.
7. If you have a disability for which you are or may be requesting
an accommodation, you are encouraged to contact both your instructor
and the Academic Success Center's Disability Resources and Services
Office (Hanley Library, Room 218) as early as possible in the term.
The ASC will verify your disability and determine reasonable accommodations
for this course. Contact Kara Kennedy, Learning Development Specialist,
at 362-7609 for more information.
Tips for Success:
1. Read the material BEFORE coming to class!! We will be covering
a lot of material relatively quickly. If you are prepared before
coming to class, your understanding of the lectures will be immensely
enhanced. Although the exams will be primarily based on what is
covered in lecture, the readings are critical for strengthening
your comprehension of the material. Avoid relying heavily on the
PowerPoint notes as your primary study tool! They only provide a
rough outline of what you are expected to understand and are meant
to complement, not replace, lecture attendance and careful note
taking and reading.
2. Review your lecture notes before the next class. I strongly
recommend re-writing them or outlining them - believe it or not,
there is a direct link between the hand and the brain! This is a
good time to coordinate what you are learning in lectures with what
you are reading in the book, BEFORE the class moves ahead to the
next topic. If there is still an area of confusion, then please
come to me for help, which brings me to my next tip....
3. DO NOT GET BEHIND!! And I don't just mean with the readings!!
Biology is built from simple principles, but eventually we will
be learning about relatively complex phenomena. If you don't understand
the basics, then you won't understand the more advanced materials.
Please come to me if there are concepts that you are really stuck
on. Which again leads to yet another point...
4. Take advantage of your instructors!! (And I don't mean that
in the negative sense.) We have office hours and discussion sessions
for a reason. And, remember, there is no such thing as a "stupid
question"! Nonetheless, please make at least some effort to
find out the answer yourself before coming for help. Part of being
a college student is learning how to find the answers on your own.
e-mail is another good way to get some help, especially for relatively
short queries.
5. Find the study aids that work best for you. Some students learn
well in informal study groups with their friends. Others prefer
aids such as flashcards, etc... A combination of all of the above
often works well. Do not waste time extensively reviewing the "easy"
stuff, focus on the material that gives you the most difficulty.
Here is where testing yourself can really help a lot, whether you
use flashcards or your friends as the testing aid!
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