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Recipes

Buffer Recipes

  • Homogenization Buffer
  • Buffer C (Sepharose 4B Column Buffer)
  • Buffer D (Cytosol Dialysis Buffer)
  • 10 X Golgi Assay Buffer Mix (Minus DTT)
  • 10 X Synaptosome Assay Buffer Mix (Minus DTT)
  • GTP Overlay Buffer
  • 4 X SDS Sample Buffer
  • 2-D Gel Equilibration Buffer
  • 10 X KHMgE (Heuser) Buffer

Gel Recipes

  • 30:0.4 Acrylaminde/bis-acrylaminde Stock
  • 4X Concentrated Running Gel Buffer
  • 4X Concentrated Stacking Gel Buffer
  • 10X Concentrated SDS-PAGE Reservoir Buffer
  • 10X Concentrated Immunoblotting TRANSFER Buffer
  • 10 X Concentrated Tris-buffered saline + Tween (TBST)
  • 6.5% gel (8 ml)
  • 6.5% gel (20 ml)
  • 8.0% gel (20 ml)
  • 10.0% gel (20 ml)
  • 11.0 % gel (20 ml)
  • 12.5 % gel (20 ml)
  • 14.0% gel (20 ml)
  • 4.0% Stacking Gel (5 ml)

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Preparations

Rat Brain Synaptosomes Preparation

  • Synaptosome Homogenization Buffer
  • 6% (w/v) FICOLL 400 In Homogenization Buffer
  • 9% (w/v) FICOLL 400 In Homogenization Buffer
  • 13% (w/v) FICOLL 400 In Homogenization Buffer

Rat Liver Lysosomes Preparation

  • Homogenization Buffer

Synaptic Plasma Membranes from Gradient-Purified Synaptosomes Preparation

  • Hypotonic Synaptosome Lysis Buffer
  • 1.7 M Sucrose Solution (48% w/w)
  • 0.92 M Sucrose Solution (28.5% w/w)

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Recipes

Preparations

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