prev next front |1 |2 |3 |4 |5 |6 |7 |8 |9 |10 |11 |12 |13 |14 |15 |16 |17 |18 |19 |20 |21 |22 |23 |24 |25 |26 |27 |28 |29 |30 |31 |32 |33 |34 |35 |36 |37 |38 |39 |40 |41 |42 |43 |44 |review

If we start at the top of the pathway, we have found that the receptor is not distributed all over the cell but rather concentrated within cilia.

This is an image taken w/ a spinning disc confocal to give a thin optical section w/o causing photobleacing.

 

Next I synthesized a version of the ligand modified by a fluorophore.

 

Binding is not static but causes a change in localization– measure patched fluorescence from pictures like thisŕ Ptc1 is dislocated from cilia.