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The map on the lower left shows the counties of PA.Our study area is in southwestern Pennsylvania, includes Allegheny county, which is highlighted in blue, and Armstrong county, which is highlighted in orange. The large map expands the view of three rivers of Allegheny county, which are Allegheny river, flows from northeast to southwest, the Monongahela river which flows north to Pittsburgh from West Virginia, and the Ohio river formed at the junction of the Allegheny and Monongahela rivers. Presented on the map are the locations of catfish catch within the Pittsburgh Pool, at the highland park dam on the Allegheny, at the U.S. Steel Edger Thompson Works on the Monongahela and at the confluence of the Allegheny and Monongahela rivers at Point State Park. We chose an upstream location at Kittanning, PA on the Allegheny River, to catch catfish because local fishers said in focus groups that they preferred to eat fish caught from this area because the water is cleaner. Kittanning is located around 36 miles upstream from Pittsburgh on the Allegheny river in Armstrong county, highlighted in orange color in the county map on the lower left. We also purchased channel catfish from local fish market for comparison purpose. These fish came from a Georgia Fish Farm.