||Figure 9. Suppressant effects of EPA on the
voltage activated L-type Ca2+current in adult rat ventricular myocytes.
Superimposed current density traces were elicited by 200 msec pulses from a
potential of –50 to 50 mV. From a holding potential 0f –80 mV, brief
depolarizations were applied to maintain a constant intracellular Ca2+ load.
To elicit a test pulse, a slow ramp from –80 to –60 mV was applied, and the
membrane potential was held at –60 mV for 50 msec before the test
depolarization was applied. ICa,L records under control conditions (open
circles), 1.5 µM EPA (filled circles),
and washout (crosses) are shown (A) from a typical cell, while averaged
normalized relationships are shown (B) for control.(n=11), 1.5
µM EPA (n=11), and washout (n=5). The
current density values were normalized to the level observed under control
condition for each experiment.