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Variations can occur during the collection, testing, storage of samples for measuring the biochemical outcomes, if they are collected differently, tested differently and by different persons. It is always wise to compare the results with some other external or internal laboratories which are standardized as external and internal controls for cross checking

Seasonal variations can also occur in risk factor levels and if not considered, will cause serious lapses in measurement. Hence collection, storage, testing of samples should be done in a standardized manner that too at the same time to reduce seasonal variations and observer.