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The concentration of spermatozoa should range from 30-800 million/ml. Second ejaculates collected 1 hour after the first will have a concentration of about 50 % of the first.

The total number of spermatozoa per ejaculate vary with the season of the year. The highest total occurs in July and lowest total in January. For example, the first ejaculate may contain an average of 22 billion spermatozoa in July and 10 billion in January. This means that early in the breeding season a stallion's total spermatozoal output may be about 50 % of his maximum capable spermatozoal output.