ANNE RAUB GREENLEE, PH.D.
Marshfield Clinic Research Foundation
1000 N. Oak Ave
Marshfield, Wisconsin 54449
(715) 389-4012 (work)
greenlee.anne@mcrf.mfldclin.edu
Education
1989 PhD Washington State University Pullman, WA
1982 MS Washington State University Pullman, WA
1973 BS University of Illinois Chicago, IL
Professional Experience
10/01 – date Director, Midwest Center for Agricultural Health and Safety, Marshfield, WI.
· Develop research agenda; supervise projects on agricultural worker health and safety.
· Represent Midwest Center at national and international scientific meetings.
· Manage personnel and budgets; report accomplishments.
12/00 – date Research Scientist, Marshfield Clinic Research Foundation, Marshfield, WI.
· Direct laboratory and epidemiology studies on agricultural exposures and effects on reproductive and neurological health.
· Research:
o Fertility risks for women working in agricultural settings.
o Developmental injury for murine embryos exposed to low-dose agrochemicals.
o Toxicity testing using murine embryonic stem cells.
o Pesticide exposure, genetic factors and risk of Parkinson Disease.
· Awarded $734,097 in extramural funding as principal investigator.
· Secured $425,000 in capital equipment (e.g. MoFloTM Cytometer; LightCyclerTM).
· Mentor to 3 undergraduates, 1 doctoral student and 1 postdoctoral fellow.
· Respond to media and lay requests for technical information.
10/96 – 12/00 Associate Scientist, Marshfield Clinic Research Foundation, Marshfield, WI
· Directed laboratory and epidemiology studies on agricultural exposures and effects on reproductive health (fertility, pregnancy, prostate cancer).
· Research:
o Altered growth factor signaling in pesticide-exposed embryos.
o Estrogenic pesticide effects on early embryonic development.
o Biomarker analyses and biorepository for prostate cancer case-control study.
o Embryo biopsy and sexing as a means to avoid X-linked diseases.
· Awarded $411,827 in extramural and internal funding as principal investigator.
· Mentored 5 undergraduate students.
1992 – 96 Research Fellow, Marshfield Clinic Research Foundation, Marshfield, WI.
1990 – 92 Postdoctoral Research Fellow, Biological Sciences, University of Idaho, Moscow, ID.
1991 Visiting Assistant Professor, Biological Sciences, University of Idaho, Moscow, ID.
1988 - 90 Postdoctoral Research Fellow, Animal Sciences, WA State University, Pullman, WA
Honors
2000 American Society for Cell Biology and Emily Mudd Fellowship to attend “Frontiers in Reproduction: Molecular and Cellular Concepts”, Marine Biology Laboratory, Woods Hole, MA
Peer-Reviewed Publications
Greenlee AR, Ellis TM, Berg RL. 2003 Low-dose agrochemicals and lawn care pesticides induce developmental injury in murine preimplantation embryos. (submitted, Environmental Health Perspectives).
Greenlee AR, Kronenwetter-Koepel T, Kaiser S, Ellis TM, Liu K. 2003 Combined effects of Matrigel and growth factors on undifferentiated murine embryonic stem cells for embryo toxicity testing, (submitted, Toxicology In Vitro).
Giampietro PF, Blank RD, Raggio CL, Merchant S, Jacobsen FS, Faciszewski T, Shukla SK, Greenlee AR, Reynolds C, Schowalter DB. 2003 Congenital and idiopathic scoliosis: clinical and genetic aspects. Clincal Medicine and Research. 1:125-136.
Greenlee AR, Arbuckle TE, Chyou PH. 2003 Risk factors for female infertility in an agricultural region. Epidemiology 14:429-436.
Greenlee AR, Burmester JK, Hiner BC. 2002 Pesticide exposure, host susceptibility factors and risk of Parkinson’s disease. WI Med J 101:23-27.
Greenlee AR, Quail CA, Berg RL. 2000. The anti-estrogen ICI 182,780 abolishes developmental injury for murine embryos exposed in vitro to o,p’-DDT. Reprod Toxicol 14:225-234.
Stueland D, Greenlee AR. 1999. Factors affecting initial cholinesterase levels from a pilot study of central Wisconsin farmers. J Ag Safety Health 5:367-371.
Greenlee AR, Quail CA, Berg RL. 1999. Developmental alterations in murine embryos exposed in vitro to an estrogenic pesticide, o,p’-DDT. Reprod Toxicol 13:555-565.
Greenlee AR, Stueland DT, Jacks JJ. 1998. Endocrine disrupting chemicals and human health implications. Marshfield Clinic Bulletin 38:3-12.
Greenlee AR, Krisher RL, Plotka ED. 1998. Rapid sexing of murine preimplantation embryos using a nested, multiplex polymerase chain reaction (PCR). Mol Reprod Dev 49:261-267.
Greenlee AR, Dodson MV, Yablonka-Reuveni Z, Kersten CA, Cloud JG. 1995. In vitro differentiation of myoblasts from skeletal muscle of rainbow trout (Oncorhynchus mykiss). J Fish Biol 46:731-747.
Greenlee AR, Kersten CA, Cloud JG. 1995. Effects of triploidy on rainbow trout myogenesis in vitro. J Fish Biol 46:381-388.
Greenlee AR, Ristow SS. 1993. A monoclonal antibody which binds a carbohydrate moiety broadly expressed on rainbow trout leukocytes. Fish and Shellfish Immunology 3:317-330.
Greenlee AR, Brown RA, Ristow SS. 1991. Nonspecific cytotoxic cells of rainbow trout kill YAC-1 targets by both necrotic and apoptic mechanisms. Devel Comp Immunol 15:153-164.
Congleton JL, Greenlee AR, Ristow SS. 1990. Isolation of leukocytes from the anterior kidney and spleen of rainbow trout in a self-generating gradient. J Fish Biol 36:575-585.
Greenlee AR, Magnuson NS, Smith C, Butt B, McKiernan AJ, Davis WC. 1990. Characterization of heteromyeloma fusion partners which promote the outgrowth of porcine hybridomas. Vet Immunol Immunopath 26:267-283.
Greenlee AR, Banks KL. 1985. Cellular and lipopolysaccharide subunit requirements for the carprine lymphoblastic response to endotoxin. Am J Vet Res 46:75-79.
Abstracts
Carl WR, Chyou P-H, Herr J, Kempf DL, Greenlee AR. 2003 Potential sources of bias in a study of infertility and environmental exposures. Annual meeting of the American College of Epidemiology. Chicago, IL.
Ellis TM, Berg RL, Mercieca MD, Greenlee AR. 2003 Pregnancy outcomes for murine embryos exposed in vitro to an estrogenic pesticide. Midwest Society of Toxicology. Lincolnshire, IL.
Koepel T, Ellis TM, Kaiser S, Greenlee AR. 2003 Characterization of murine embryonic stem cells cultured on Matrigel in a feeder-free and conditioned medium-free environment. Keystone Conference. Steamboat Springs, CO.
Greenlee AR, Arbuckle TE, Chyou PH. 2002 Risk factors for female infertility in an agricultural region. 14th Conference of the International Society for Environmental Epidemiology, Vancouver, BC. Epidemiology 13:S247.
Greenlee AR, Ellis TM, Berg RL. 2002 Developmental injury in murine preimplantation embryos exposed in vitro to commonly used agrochemicals. 42nd Annual Meeting Teratology Society, Scottsdale, AZ. Teratology 65:310.
Greenlee AR, Quail CA, Berg RL, Linneman J, Mercieca MD. 2000. Pregnancy outcomes for murine embryos exposed in vitro to the estrogenic pesticides, o,p’-DDT. Fertil Steril 74:S133-S134.
Greenlee AR, Quail CA, Misner LM, Liu K: 2000 Insulin-like growth factor expression in murine embryos exposed in vitro to o,p’-DDT. 39th Annual Meeting of the Society of Toxicology, March 19-23, 2000, Philadelphia, PA. Toxicol Sci 2000;54:S289.
Greenlee AR, Quail CA, Berg RL: 1999 The anti-estrogen ICI 182,780 abolishes developmental injury for murine embryos exposed in vitro to the estrogenic pesticide o,p’-DDT. American Society for Reproductive Medicine, September 25-30, 1999, Toronto, Canada. Fert Steril 1999;72:S16.
Stueland DT, Greenlee AR: 1998 Factors affecting initial cholinesterase levels from a pilot study of farmer acceptance in central Wisconsin. Presented at the Fourth International Symposium: Rural Health and Safety in a Changing World, October 18-22, 1998, p 017, Saskatoon, Saskatchewan, Canada.
Greenlee AR, Berg RL: 1998 Developmental changes in murine embryos exposed in vitro to an estrogenic pesticide, o,p’-DDT. Presented at the American Society for Reproductive Medicine, October 4-9, 1998, San Francisco, CA.
Greenlee AR, Krisher RL, ED Plotka: 1996 Rapid sexing of murine preimplantation embryos using a nested, multiplex polymerase chain reaction (PCR). American Society for Reproductive Medicine. 52nd Annual Meeting, November 2-7, 1996, Boston, MA. Fertility and Sterility O-16; S9.
Greenlee AR, Plotka ED: Endotoxin induced embryotoxicity is not intensified by cumulus co-culture. Presented: 50th American Fertility Society Meeting, November 5-10, 1994, San Antonio, TX. Fertility and Sterility Supplement, S179-180.
Greenlee AR, Dodson MV, Yablonka-Reuveni Z, Kersten C, Cloud JG: Culture conditions facilitating differentiation of trout myogenic stem cells. Presented: Gordon Research Conference, “Myogenesis” June 15-19, 1992, Tilton, New Hampshire.
Book Chapter
Greenlee AR and Ristow SS. 1992. Detection of apoptic killing by trout nonspecific cytotoxic cells using the DNA fragmentation assay and agarose gel electrophoresis. In: Techniques in Fish Immunology. Eds: JS Stolen, TC Fletcher, DP Anderson, SL Kaattari and AF Rowley. SOS Publications, Fair Haven, NJ.
Professional Memberships
2002 International Society for Environmental Epidemiology
2002 Teratology Society
2001 Society for the Study of Reproduction
1999 The Society of Toxicology
1998 Midwest Regional Chapter of the Society of Toxicology
1995 American Association of Bioanalysts
1992 American Society for Reproductive Medicine
Committees
2003 – date USDA-ARS Institute for Environmentally Integrated Dairy Management
2003 – date Associate Editor; Clinical Medicine and Research
1999 – date Institutional Animal Care and Use Committee
1999 – 2002 Institutional Research Committee
1996 – 1999 Wisconsin Pesticide Sales and Use Database
1994 – 1998 Co-Chair and Chair; Institutional Review Board
Invited Presentations
02/04 “Pesticides, Genetics and Risk of Parkinson’s Disease”. Science, Technology, Health Care and Science Teachers, Marshfield, WI.
07/03 “Stem Cells – Sources, Controversy, and the Future of Replacement Parts”. Biology of Human Reproduction and Sexuality, UW-Wood County, Marshfield, WI.
07/02 “Screening of Agricultural and Lawn Care Pesticides for Developmental Toxicity Using the Mouse Embryo Assay”. SOEH International Conference on Pesticide Use and Health, NIH, Bethesda, MD.
02/02 “Stem Cells-Sources, Controversy, Tissue Regeneration & Research Applications”. Science, Technology, Health Care and Science Teachers, Marshfield, WI.
10/01 “Environmental Toxins and Their Effects on Fertility”. American Society for Reproductive Medicine, Orlando, FL
06/01 “Protecting the Most Vulnerable”. Stueland Summer Symposium, Marshfield, WI
04/01 “Evaluation of pesticide-induced developmental injury using the mouse embryo assay”, Department of Cell Biology and Neuroscience, University of South Carolina School of Medicine, Columbia, SC.
04/00 “Screening of developmental toxicants using the mouse preimplantation embryo assay". Environmental Toxicology 800: Current Research in Environmental Toxicology course, University of Wisconsin, Madison, WI.
Postgraduate Studies
10/03 Clinical proteomics: Translational applications using laser capture microdissection, NIH, Bethesda, MD.
8/02 Environmental agents and human reproduction: overcoming methodologic challenges. Vancouver, BC.
6/02 Mechanisms, Biomarkers, Data Interpretation: An Integrative Risk Assessment using Developmental Neurotoxicity as a Model. Scottsdale, AZ.
12/01 Symposium on Gene Expression and Proteomics in Environmental Health Research, Natcher Center Auditorium, NIH, Bethesda, MD.
10/01 The Role of Environmental Toxicants in Premature Birth, National Academy of Sciences, Washington, DC.
References
Michael D. Caldwell, M.D., Ph.D James K. Burmester, PhD
Director, Marshfield Clinic Research Foundation Senior Research Scientist
1000 N. Oak Ave 1R3 Marshfield Clinic Research Foundation
Marshfield, WI 54449 1000 N. Oak Ave 2R6
(715) 387-5107 (Ph) Marshfield, WI 54449
caldwell.michael@mcrf.mfldclin.edu (715) 389-4368 (Ph)
burmester.james@mcrf.mfldclin.edu
Barbara C. Lee, RN, PhD
Director, National Farm Medicine Center
1000 N Oak Ave ML1
Marshfield, WI 54449
(715) 389-9182 (Ph)