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Results: An asbestos-related occupation or hobby of a household member and use of flea repellents on the dog were significantly associated with mesothelioma. In addition, there was a trend indicating an increased risk of mesothelioma with an urban residence. Lung tissue from three dogs with mesothelioma and one with squamous cell carcinoma of the lung had significantly higher concentrations of chrysotile asbestos fibers than lung tissue of age-matched control dogs that died of non-pulmonary related causes. These findings confirm that asbestos exposure is a risk factor for mesothelioma in the dog, as it is in humans.