I am working on experiment which requires me to apply the repeated measures two-way ANOVA analysis model. It involves comparing the effects of 5 to 7 treatments, normal control, and +ve control on weekly measured parameters over 6 weeks experimental period. Now, I hope if you would guide me to the right way to graphically represent the significance on the two- way analysis charts, as I know the two-way ANOVA should be graphically illustrated using the line chart type, but where should I position the significance marks (stars)? We have utilized Statgraphics Centurion 16.1.11, and the GraphPad prism version 6 software for the statistical evaluation. In the two-way repeated measures model, both programs have indicated the overall groups' significance i.e. they indicated for example that the +ve control is significant from normal, or the treatment no 1 is significant from the +ve control. Both the Statgraphics and the Graphpad prism have indicated the same results. They didn’t indicate at which time point (week) the results were significant and at which week they were not. Should I position the stars on all the time points as indicated by the two-way ANOVA results? Or should I position them on the significant weeks only based on further one-way ANOVA analysis for the results of each week separately?