Histology lab 3: Jan. 25–26, 2005

Muscle (Smooth, Cardiac & Skeletal) and Nervous Tissue (Spinal Cord & PNS, Visceral Sensory Cells, and Motor-End Plates)

collection slide #
label description
Muscle   
Epithelia/Endocrine/Muscle 3513 Muscle composite: sec three sections: skeletal, cardiac, smooth: sarcomeres, striations (not on smooth), intercalated disks (cardiac), corkscrew nuclei (smooth); note position of nuclei and cell (syncytium) size
Epithelia/Endocrine/Muscle 3533 Heart Purkinje fibers: sec conduction fibers beneath endocardium, large, empty cells; note intercalated disks
Epithelia/Endocrine/Muscle 4059 Heart rat: c.s. epicardium, myocardium, endocardium; note intercalated disks
Epithelia/Endocrine/Muscle 3527 Cardiac muscle: thin sect: IH. intercalated disks and mitochondria stain well with iron hematoxylin
Digestive System
4499, 4508, 4526, 4530, 4534, 4542, or 4558
Esophagus, stomach, intestine (various), or recto-anal junction
smooth muscle in outer and inner layers (muscularis externa)  (as well as muscularis mucosae); note corkscrew nuclei
Urogenital/Reproductive
7303 or 7306
Uterus human: fol. phase: or progravid phase
smooth muscle in bundles
Integ/Respir
7018
Human scalp white: v.s.
arrector pili
Circulatory/Immune/Hematopoiesis
4022, 6516, or 8354
artery or vein
smooth muscle in layers (media)
CT 3539 Muscle-tendon connection: sec  muscle fibers (red); collagenous bundles (orange); striations on oblique sections of muscle
Nervous Tissues (CNS)   
Nerve 3627 Neurofibrillae Nerve cells: sec perikaryons, dendrites (branched; tapering), axons (unbranched, nearly uniform diameter)
Nerve
3635
Neuroglia cells & fibers: sec
neuroglial stain is not especially specific but you should see fibrous astorcytes with vascular pedicels in this section of brain; chromatophilic substance (Nissl's bodies) absent in axon hillock and axon
Nerve 3703 Spinal cord: c.s & l.s. compare white and gray matter, dorsal (posterior) median sulcus; ventral (anterior) median fissure
Nerve 3778 Optic nerve mammal: c.s. epineurium/perineurium, endoneurium, axons of ganglion cells, myelin from oligodendrocytes
Nerve 8288 Spinal cord primate: c.s. gray and white matter; dorsal (posterior) & ventral (anterior) horns; motor nerve (ventral horn); pia mater; central canal; ependyma
Nervous Tissues (PNS)
Nerve
3651
Medullated nerves
myelin sheath, axons; neurofibral node (of Ranvier), neurolemmacytes (Schwann cells),  endoneurium, perineurium, epineurium
Nerve
3657
Motor Nerve Endings: w.m.
motor end plates and axons; myelinated fibers and unmyelinated portions near end plates.
Nerve 3696
Spinal ganglion & dorsal nerve root: c.s.
dorsal (posterior) root; (unipolar) sensory nerves, satellite cells
Nerve
3715
Sympathetic ganglion
post ganglionic nerve cell bodies surrounded by satellite cells
Digestive System
4526
Duodenum: c.s.
myenteric plexus (of Auerbach); submucous plexus (of Meissner); satellite cells
Sensory Cells/structures
Nerve
3659
Muscle spindle: w.m.
stretch receptors (basket & chain types): capsule, intrafusal fibers, extrafusal fibers, perimysium); unmyelinated nerve fibers
Integum/Respiratory, also Digestive System
3663 also 4600
Pacinian corpuscle; Pancreas: sec
(deep pressure) lamellated, corpuscular organ, unmyelinated root of nerve
Digestive System
4458 or 4466
Taste buds tongue rabbit: v.s.,  Vallatepapillae: v.s.
gustatory (sensory cells) supported in taste bud by sustentacular cells and resting on basal cells; microvilli (taste hairs) in taste pore
Integum/Respiratory
8280
Meissner's corpuscles primate: sec
tactile corpuscle, unmyelinated root of nerve, myelinated peripheral nerves

Last update: Nov. 22, 05
E-Mail Address: sshostak+@pitt.edu