Histology lab 2: Jan. 18–19, 2005 

Connective Tissue, Cartilage, Bone, Adipose Tissue and Blood

collection slide #
label description

loose irregular
CT 3224 Areolar tissue spread film collagen fibers (ribbons), elastin fibers (taunt strings), macrophages (histiocytes), fibroblasts, mast cells, plasma cells
CT
3236
Reticular tissue thin sec M & NFR
fine reticular fibers encircling lymph nodules
CT
3240
Mast cells mesentery: resor fuch/H
mast cells, fibroblasts, macrophages (histiocytes), plasma cells
CT
4891

Lung elastic fibers: sec
elastic fibers; arteries & arterioles: fenestrated sheets (laminas) & elastic fibers
CT
6105
Umbilical cord: c.s dry embryonic connective tissue (Wharton's jelly)
Cir Sys/Hemato
6516
Artery human: c.s.
fenestrated sheets (laminas) & elastic fibers

loose/dense irregular (also stroma of glands: capsule and trabeculae or septa)
Integum/ Respiratory
7015, 7018 or 7034
Human skin, scalp, or axilla
papillary/reticular portion of dermis
Uro/Endocrine
5453 or 5454
Testis
tunica albuginea and (interlobular) septa

dense regular
CT 3259 Ligamentum nuchae: ls elastic fibers
CT
3539
Muscle-tendon connection: sec collagen fibers (note corrugation) fused to skeletal muscle
  cartilage
CT 3264 Hyaline cartilage: sec perichondrium: fibrous & chondrogenic layers; chondroblasts; isogenous colonies; lacunae, chondrocytes, fat globules; matrix (interterritorial, territorial)
CT 3271 Elastic cartilage: sec perichondrium; chondroblasts; chondrocytes; elastic fibers in matrix
CT 3275 Elastic cartilage epiglottis: sec. perichondrium: fibrous & chondrogenic layers; chondroblasts; chondrocytes; elastic fibers in matrix
CT 3280 White fibro-cartilage; Pubic symphysis: sec no perichondrium; chondrocytes vaguely lined up between collagenous bundles

bone (identify bone by color and texture; skip ossification)
CT 3283 Membranous bone fatal skull: sec blue matrix around teeth; osteoblasts, osteocytes, osteoclasts
CT 3291 Compact bone decalcified cs orange matrix; laminae; osteoblasts; osteoclasts; 
CT 6140 Bone dry ground human: c.s. compact bone: do not use high power: osteon systems: laminae around central and perforating canals; lacunae of osteocytes; canaliculli
CT 6143 bone dry ground human: l.s. compact bone: do not use high power: osteon systems: laminae around central and perforating canals; interstitial lamellae, cement lines, lacunae of osteocytes; canaliculli
  adipose
CT
3232
Adipose tissue Osmic acid: sec.
unilocular adipocytes (look at periphery of section)
CT
3234
Brown adipose tissue mammal: sec.
multilocular adipocytes (find well-fixed cells at periphery

blood
CT
6540

Human blood smear: Wright
Red blood cells (RBCs); Lymphcytes; White blood cells (leucocytes): Granulocytes: neutrophils (also called polymorphonuclear leucocytes or polys), basophils, eosinophils; Agranulocytes: monocytes; Platelets

Last Updated: Nov. 22, 2004
E-Mail Address: sshostak+@pitt.edu