HomeOverviewPeptide CostsCertified PeptidesStaff and Contact Info.Order Form

 

Peptide Synthesis

The Peptide Synthesis Facility is a core of ctsi, and provides comprehensive services for peptide synthesis, purification and characterization to research programs at the University of Pittsburgh and UPMC. External requests for peptide production may be considered. Peptide synthesis is performed by one of the following: Liberty Microwave Synthesizer, aaaptec 396 Omega Multiple Biomolecular Synthesizer , Applied Biosystems 433A Peptide Synthesizer.

Typically, synthesis begins with the protected amino acid being covalently anchored to an insoluble polystyrene resin through the carboxylic acid. At the end of the synthesis, the peptide is cleaved, extracted and purified by gel filtration. Analytical HPLC is performed for mass spectrometry results and to determine the purity of the final product. The actual weight of the product and its degree of purity will depend on the scale and the sequence of the peptide.