Laboratory
Techniques:
Protein Chemistry: differential detergent fractionation; non-detergent
protein separation techniques; sucrose gradient membrane protein preparation;
nuclear protein preparation; quantification of protein concentration by BCA
assay; SDS-PAGE gel electrophoresis; Western blotting; immunoprecipitation
Molecular Biology: DNA and RNA preparation and purification;
quantification of DNA and RNA concentration by spectrophotometer; DNA and RNA
gel electrophoresis; plasmid mini and maxi preps; gel extraction; plasmid
transformation; Southern blotting; Northern blotting; PCR; RT-PCR; real-time PCR
Histochemistry:
H&E staining; immunohistochemistry; immunofluorescence; bright field
microscopy; fluorescence microscopy; confocal microscopy (all on both
fixed-frozen and paraffin-embedded tissues)
Clinical Chemistry: cholesterol,
triglyceride, and glucose assays; processing and freezing blood serum and plasma
samples
Other Techniques: animal survival surgeries (retro-orbital bleeding,
tail clipping); non-survival surgeries (organ perfusion); short-term primary
cell culturing; long-term primary cell culturing; bioassays; siRNA transfection
into primary cell cultures; determining viability and yield through cell counts;
film developing; solution preparation
Familiar with: zymography; ELISA; DNA dot blots
Authorship:
Yang
J, Shultz RW, Mars WM, Wegner RE, Li Y, Dai C, Nejak K, Liu Y.
Disruption
of tissue-type plasminogen activator gene in mice reduces renal interstitial
fibrosis in obstructive nephropathy.
J
Clin Invest. 2002 Nov;110(10):1525-38.
Acknowledgements:
Lagoa
CE, Vodovotz Y, Stolz DB, Lhuillier F, McCloskey C, Gallo D, Yang R, Ustinova E,
Fink MP, Billiar TR, Mars WM.
The
role of hepatic type 1 plasminogen activator inhibitor (PAI-1) during murine
hemorrhagic shock.
Hepatology.
2005 Aug;42(2):390-9.
Schoedel
KE, Tyner VZ, Kim TH, Michalopoulos GK, Mars WM.
HGF,
MET, and matrix-related proteases in hepatocellular carcinoma, fibrolamellar
variant, cirrhotic and normal liver.
Mod
Pathol. 2003 Jan;16(1):14-21.
Ma Z, Thomas KS, Webb DJ, Moravec R, Salicioni AM, Mars WM, Gonias SL.
Regulation
of Rac1 activation by the low density lipoprotein receptor-related protein.
J
Cell Biol. 2002 Dec 23;159(6):1061-70. Epub 2002 Dec 23.
Monga
SP, Mars WM, Pediaditakis P, Bell A, Mule K, Bowen WC, Wang X, Zarnegar R,
Michalopoulos GK.
Hepatocyte
growth factor induces Wnt-independent nuclear translocation of beta-catenin
after Met-beta-catenin dissociation in hepatocytes.
Cancer
Res. 2002 Apr 1;62(7):2064-71.
Powell
EM, Mars WM, Levitt P.
Hepatocyte
growth factor/scatter factor is a motogen for interneurons migrating from the
ventral to dorsal telencephalon.
Neuron.
2001 Apr;30(1):79-89.
Abstracts:
Mars, W.M., Nejak, K., Alderson, A.-M., Gong, D., Hsu, Y-M, Jakubowski, A. & Burkly, L.C.
TWEAK
directly induces death of primary cultured rat hepatocytes. FASEB summer
conference 2002