Protocols - Entire Phagehunting Procedure Manual from finding a phage to its GenBank Submission. Current protocols are under review. Click here for entire manual or select a specific procedure from the Table of Contents.

Table of Contents

Introduction

Checklists

Student Agreement

Summary Report Requirements

Background

Division M: Bacteriophages (from ASM)

Phage Genome Analysis Background

Phage Facts

Media

Growing M. smegmatis mc2 155

Growing M. smegmatis mc2 4518

Isolation & Purification

Outline

Outline Procedural Notes

Procedure for Harvest, Precipitation, and Dialyzation of Phage

DNA Preparation

Restriction Enzyme Analysis and Electrophoresis of DNA

EM Grid Preparation

Genome Center Protocols

Mini-Prep Manufacturer's Protocol

Protein Electrophoresis

Lysogen Protocol

End Determination

Annotation

BLASTing

GenBank Submission

Appendices

Saftey Notes

University Policy for Ethidium Bromide Disposal

Math & Dilutions

Density and Refractive Index

Hatfull Lab Guidelines

Visitor's Guide to the Hendrix Lab

Genome Center Guidelines

 

Assessment Tools

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