Elodie Ghedin, Ph.D

Elodie Ghedin

Biography
Selected Publications
Contact Information

Biography

Elodie Ghedin, Ph.D. is an Assistant Professor in the Division of Infectious Diseases since May 2006. She earned her B.S. and Ph.D. from McGill University in Canada and was a postdoctoral fellow at the National Institute of Allergy and Infectious Diseases (NIAID) (1998-2000). The underlying theme in Dr. Ghedin’s research is the genomics of infectious diseases. She has worked on the causative agents for African sleeping sickness, Chagas disease, cutaneous and visceral leishmaniases, and lymphatic filariasis. During her time at The Institute for Genomic Research (TIGR) Dr. Ghedin led the Viral Genomics group, working on the design of high throughput pipelines for virus discovery and characterization. Her laboratory was the first to produce an efficient and rapid workflow for the systematic decoding of complete influenza genomes. Ongoing projects in Dr. Ghedin’s laboratory include virus detection in juvenile-onset diabetes patients, gene structure characterization in a parasitic nematode (Brugia malayi), the evolution of retrotransposons in trypanosomatid parasites and the study of drug resistance evolution in influenza. Dr. Ghedin maintains an Adjunct Investigator position at TIGR where she spent the last 6 years.




Selected Publications

  1. Yao K, Mandel M, Akyani N, Maynard K, Sengamalay N, Fotheringham J, Ghedin E., Kashanchi F, Jacobson S. (2006) Differential HHV-6A gene expression in T cells and primary human astrocytes based on multi-virus array analysis. Glia. Jun;53(8):789-98.

  2. Bringaud F, Bartholomeu DC, Blandin G, Delcher A, Baltz T, El-Sayed NM, Ghedin E. (2006) The Trypanosoma cruzi L1Tc and NARTc non-LTR retrotransposons show relative site specificity for insertion. Mol Biol Evol. Feb;23(2):411-20.

  3. Bringaud F, Ghedin E, Blandin G, Bartholomeu DC, Caler E, Levin MJ, Baltz T, El-Sayed NM. (2006) Evolution of non-LTR retrotransposons in the trypanosomatid genomes: Leishmania major has lost the active elements. Mol Biochem Parasitol. Feb;145(2):158-70.

  4. Ghedin E, Sengamalay NA, Shumway M, Zaborsky J, Feldblyum T, Subbu V, Spiro DJ, Sitz J, Koo H, Bolotov P, Dernovoy D, Tatusova T, Bao Y, St George K, Taylor J, Lipman DJ, Fraser CM, Taubenberger JK, Salzberg SL. (2005) Large-scale sequencing of human influenza reveals the dynamic nature of viral genome evolution. Nature. Oct 20;437(7062):1162-6.
  5. Holmes EC, Ghedin E, Miller N, Taylor J, Bao Y, St George K, Grenfell BT, Salzberg SL, Fraser CM, Lipman DJ, Taubenberger JK. (2005) Whole-genome analysis of human influenza A virus reveals multiple persistent lineages and reassortment among recent H3N2 viruses. PLoS Biol. Sep;3(9):e300.

  6. Ghedin E, Claverie JM. (2005) Mimivirus relatives in the Sargasso sea. Virol J. Aug 16;2:62.

  7. Berriman M, Ghedin E, Hertz-Fowler C, Blandin G, Renauld H, Bartholomeu DC, Lennard NJ, Caler E, Hamlin NE, Haas B, Bohme U, Hannick L, Aslett MA, Shallom J, Marcello L, Hou L, Wickstead B, Alsmark UC, Arrowsmith C, Atkin RJ, Barron AJ, Bringaud F, Brooks K, Carrington M, Cherevach I, Chillingworth TJ, Churcher C, Clark LN, Corton CH, Cronin A, Davies RM, Doggett J, Djikeng A, Feldblyum T, Field MC, Fraser A, Goodhead I, Hance Z, Harper D, Harris BR, Hauser H, Hostetler J, Ivens A, Jagels K, Johnson D, Johnson J, Jones K, Kerhornou AX, Koo H, Larke N, Landfear S, Larkin C, Leech V, Line A, Lord A, Macleod A, Mooney PJ, Moule S, Martin DM, Morgan GW, Mungall K, Norbertczak H, Ormond D, Pai G, Peacock CS, Peterson J, Quail MA, Rabbinowitsch E, Rajandream MA, Reitter C, Salzberg SL, Sanders M, Schobel S, Sharp S, Simmonds M, Simpson AJ, Tallon L, Turner CM, Tait A, Tivey AR, Van Aken S, Walker D, Wanless D, Wang S, White B, White O, Whitehead S, Woodward J, Wortman J, Adams MD, Embley TM, Gull K, Ullu E, Barry JD, Fairlamb AH, Opperdoes F, Barrell BG, Donelson JE, Hall N, Fraser CM, Melville SE, El-Sayed NM. (2005) The genome of the African trypanosome Trypanosoma brucei. Science. Jul 15;309(5733):416-22.

  8. El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B. (2005) The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science. 2005 Jul 15;309(5733):409-15.
  9. 9. El-Sayed, N.M.A., Myler, P.J., Blandin, G., Berriman, M., Crabtree, J., Aggarwal, G., Caler, E., Renauld, H., Worthey, E.A., Hertz-Fowler, C., Ghedin, E., Peacock, C., Haas, B., Angiuoli, S., Anupama, A., Bartholomeu, D., Cadag, E., Carlton, J.M., Creasy, T., Djikeng, A., Hauser, C., Ivens, A.C., Kummerfeld, S., Leal, J., Nilsson, D., Peterson, J., Shallom, J., Silva, J.C., Sundaram, J., Tran, A.-N., Westenberger, S., White, O., Wortman, J., Melville, S.E., Donelson, J.E., Andersson, B., Stuart, K.D., and Hall, N. (2005) Comparative genomics of the trypanosomatids. Science Jul 15;309(5733):404-409.
  10. Huete-Perez JA, Flores-Obando RE, Ghedin E, Caffrey CR. (2005) Genomic and proteomic approaches for Chagas' disease: critical analysis of diagnostic methods. Expert Rev Mol Diagn. Jul;5(4):521-30.
  11. Foster, J, Ganatra, M, Kamal, I, Ware, J, Makarova, K, Ivanova, N, Bhattacharyya, A, Kapatral, V, Kumar, S, Posfai, J, Vincze, T, Ingram, J, Moran, L, Lapidus, A, Omelchenko, M, Kyrpides, N, Ghedin, E, Wang, S, Goltsman, E, Joukov, V, Ostrovskaya, O, Tsukerman, K, Mazur, M, Comb, D, Koonin, E, Slatko, B. (2005) The Wolbachia Genome of Brugia malayi: Endosymbiont Evolution within a Human Pathogenic Nematode. PLoS Biol. Mar 29;3(4):e121 [Epub ahead of print]
  12. Ghedin E, Fraser CM. (2005) A virus with big ambitions. Trends Microbiol. Feb;13(2):56-7.

  13. Porter-Kelley JM, Gerald NJ, Engel JC, Ghedin E, Dwyer DM. (2004) LdARF1 in trafficking and structural maintenance of the trans-Golgi cisternal network in the protozoan pathogen Leishmania donovani. Traffic. Nov;5(11):868-83.

  14. Guiliano DB, Hong X, McKerrow JH, Blaxter ML, Oksov Y, Liu J, Ghedin E, Lustigman S. (2004) A gene family of cathepsin L-like proteases of filarial nematodes are associated with larval molting and cuticle and eggshell remodeling. Mol Biochem Parasitol. Aug;136(2):227-42.
  15. Ghedin E, Pumfery A, de la Fuente C, Yao K, Miller N, Lacoste V, Quackenbush J, Jacobson S, Kashanchi F. (2004) Use of a multi-virus array for the study of human viral and retroviral pathogens: gene expression studies and ChIP-chip analysis. Retrovirology. May 25;1(1):10.

  16. Ghedin E, Wang S, Foster JM, Slatko BE. (2004) First sequenced genome of a parasitic nematode. Trends Parasitol. Apr;20(4):151-3.

  17. Ghedin E, Bringaud F, Peterson J, Myler P, Berriman M, Ivens A, Andersson B, Bontempi E, Eisen J, Angiuoli S, Wanless D, Von Arx A, Murphy L, Lennard N, Salzberg S, Adams MD, White O, Hall N, Stuart K, Fraser CM, El-Sayed NM. (2004) Gene synteny and evolution of genome architecture in trypanosomatids. Mol Biochem Parasitol. Apr;134(2):183-91.

  18. Seelamgari, A., Maddukuri, A., Berro, R., de la Fuente, C., Kehn, K., Deng, L., Dadgar, S., Bottazzi, M.E., Ghedin, E., Pumfery, A., and Kashanchi, F. (2004) Role of viral regulatory and accessory proteins in HIV-1 replication. Front. Biosci. 9:2388-2413.

  19. Kehn, K., Berro, R., de la Fuente, C., Strouss, K., Ghedin, E., Dadgar, S., Bottazzi, M.E., Pumfery, A., and Kashanchi, F. (2004) Mechanisms of HTLV-I transformation. Front. Biosci. 9:2347-2372.
  20. Bringaud, F., Biteau, N., Zuiderwijk, E., Berriman, M., El-Sayed, N.M., Ghedin, E., Melville, S.E., Hall, N., Baltz, T. (2004) The ingi and RIME non-LTR retrotransposons are not randomly distributed in the genome of Trypanosoma brucei. Mol. Biol. Evol. 21:520-528.

  21. El-Sayed, N.M.A., Ghedin, E., Song, J., MacLeod, A., Bringaud, F., Larkin, C., Wanless, D., Peterson, J., Hou, L., Taylor, S. Tweedie, A., Biteau, N., Khalak, H.G., Lin, X., Mason, T., Hannick, L., Caler, E., Blandin, G., Bartholomeu, D., Simpson, A.J., Kaul, S., Zhao, H., Pai, G., Van Aken, S., Utterback, T., Haas, B., Koo, H.L., Umayam, L., Suh, B., Gerrard, C., Leech, V., Qi, Q., Schwartz, D., Feldblyum, T., Salzberg, S., Tait, A., Turner, M.R., Ullu, E., White, O., Melville, S., Adams, M.D., Fraser, C.M., Donelson, J.E. (2003). The sequence and analysis of Trypanosoma brucei chromosome II. Nucl. Acids. Res. 31:4856-4863.

  22. Bringaud, F., Garcia-Perez, J. L., Heras, S. R., Ghedin, E., El-Sayed, N.M., Andersson, B., Baltz, T., & Lopez, M. C. (2002). Identification of non-autonomous non-LTR retrotransposons in the genome of Trypanosoma cruzi. Mol. Biochem. Parasitol. 124:73-78.
  23. Shakarian, A.M., Joshi, M.B., Ghedin, E., Dwyer, D.M. (2002) Molecular dissection of the functional domains of a unique, tartrate-resistant, surface membrane acid phosphatase in the primitive human pathogen, Leishmania donovani. J. Biol. Chem. 277:17994-18001.

  24. Ghedin, E., Debrabant, A., Engel, J.C., and Dwyer, D.M. (2001) Secretory and endocytic pathways converge in a dynamic endosomal system in a primitive protozoan. Traffic 2:175-188.

  25. Debrabant, A., Ghedin, E., and Dwyer, D. (2000) Dissection of the functional domains of the Leishmania surface membrane 3’-Nucleotidase/Nuclease, a unique member of the class I nuclease family. J Biol Chem. 275:16366-16372.

  26. Ghedin, E., Charest, H., Zhang, W.W., Debrabant, A., Dwyer, D., and Matlashewski, G. (1998) Inducible expression of suicide genes in Leishmania donovani amastigotes. J Biol Chem. 273: 22997-23003.

  27. Ghedin, E., Charest, H. and Matlashewski, G. (1998) A2rel: a constitutively expressed Leishmania gene linked to an amastigote-stage-specific gene. Mol Biochem Parasitol. 93: 23-29.
  28. Ghedin, E., Charest, H., Zhang, W.W., Sundar, S., Kenney, R.T. and Matlashewski, G. (1997) Antibody response against a Leishmania donovani amastigote-stage-specific protein in patients with visceral leishmaniasis. Clinical and Diagnostic Laboratory Immunology 4:530-535.

  29. Zhang, W.W., Charest, H., Ghedin, E. and Matlashewski, G. (1996) Identification and overexpression of the A2 amastigote-specific protein in Leishmania donovani. Mol Biochem Parasitol. 78:79-90.



Contact Information

Scaife Hall, Room 634
Pittsburgh, PA 15261
Telephone: 412-383-5850
Facsimile: 412-383-5851
Email: GhedinE@dom.pitt.edu  

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