Department of Orthopedic Surgery	LIVE CELL IMAGING LAB
	Stem Cell Research Center	McGowan Institute for Regenerative Medicine

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Steven M. Chirieleison, B.S. Research Specialist 5106 Rangos Research Center 3705 Fifth Avenue Pittsburgh, PA, USA 15260 schirieleison@gmail.com Education B.S. University of Pennsylvania, Bioengineering, 2007 Post-Bac University of Pittsburgh, Organic Chemistry, 2008
Current Research Human muscle-derived stem cell characterization & regeneration in dystrophic animals Optimizing cell culture conditions for hMDSCs In vitro and in vivo myogenic differentiation of human umbilical cord cells Peer-Reviewed Publications 1. Chirieleison SM , Schugar RC, Deasy BM. 2008. Current Progress in Cell-mediated Gene Therapy for Muscular Dystrophies. Research Advances in Gene Therapy. Global Research Network; Kerala, India. Mohan RM (Ed.). IN PRESS. 2. Schugar RC, Schmidt BT, Wescoe KE, Chirieleison SM , Askew Y, Nance JJ, Evron JM, Deasy BM. 2008. Primitive Mesenchymal-like Stem Cells from Human Umbilical Cord Stroma: High Isolation Yield, High Expansion and Phenotype Stability. IN REVIEW 3. Chirieleison SM , Feduska JM, Schugar RC, Sanford SL, Scelfo CC, Askew Y, Deasy BM. 2008. Human Muscle-Derived Cells Isolated by Differential Adhesion Rates Exhibit Differences in Phenotype and Contribution to Skeletal Muscle Regeneration in mdx/SCID model. IN FINAL PREP Abstract Publications 1. Chirieleison SM , Schugar RC, Schmidt BT, Deasy BM. 2009. Viability of Human Umbilical Cord as Alternative Cell Source for Muscle-Directed Therapy. Orthopaedic Research Society Annual Meeting; Las Vegas, NV, USA. PODIUM 2. Weiss C, Plassmeyer J, Witt M, Deasy BM, Chirieleison SM, Huard J, Ambrosio F. 2009. Paracrine Influences of Normal Muscle Derived Stem Cells Enhance the Myogenic Potential of Stem Cells Isolated from Dystrophic Muscles. Orthopaedic Research Society Annual Meeting; Las Vegas, NV, USA. POSTER 3. Chirieleison SM , Scelfo CC, Askew Y, Deasy BM. 2008. Development of Morphological Measurement Scheme Using Open Source Software for Live Cell Imaging Approaches to Stem Cell Biology. University of Pittsburgh Science 2008; Pittsburgh, PA, USA. POSTER 4. Ding Z, Gau D, Deasy BM, Chirieleison SM , Roy P. 2008. Ligand interactions of profilin-1 in migration and capillary morphogenesis of vascular endothelial cells. American Society for Cell Biology Annual Meeting; San Francisco, CA, USA. POSTER. 5. Chirieleison SM , Scelfo CC, Deasy BM. 2008. Quantitative Analysis of Therapeutic Muscle Cell Populations through Live Automated Cell Imaging. American Society for Cell Biology Annual Meeting; San Francisco, CA, USA. POSTER 6. Evron JM, Schugar RC, Chirieleison SM , Nance JJ, Deasy BM. 2008. The Potential of FGF-2 in the Chondrogenic Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells. Children’s Hospital of Pittsburgh Research Symposium; Pittsburgh, PA, USA. POSTER 7. Weiss C, Plassmeyer J, Witt M, Deasy BM, Chirieleison SM , Huard J, Ambrosio F. 2008. Paracrine Influences of Normal Muscle Derived Stem Cells Enhance the Myogenic Potential of Stem Cells Isolated from Dystrophic Muscles. Children’s Hospital of Pittsburgh Research Symposium; Pittsburgh, PA, USA. POSTER 8. Chirieleison SM , Scelfo CC, Askew Y, Deasy BM. 2008. Development of Morphological Measurement Scheme Using Open Source Software for Live Cell Imaging Approaches to Stem Cell Biology. Pittsburgh Orthopaedic Journal; Pittsburgh, PA, USA. 9. Scelfo CC, Chirieleison SM , Deasy BM. 2008. Integration and Adaptation of Live Cell Image Analysis. Pittsburgh Orthopaedic Journal; Pittsburgh, PA, USA. 10. Chirieleison SM , JM Feduska, RC Schugar, SL Sanford, J Huard, BM Deasy. 2008. Identifying Populations of Human Muscle Derived Stem Cells to Participate in Skeletal Muscle Regeneration Based on Phenotypic Differences. Pittsburgh Orthopaedic Journal; Pittsburgh, PA, USA. 11. Chirieleison SM , Feduska JM, Schugar RC, Witt AM, Deasy BM. 2008. In Vitro Aging of Human Muscle Stem Cells Due to Culture Expansion. International Society for Stem Cell Research; Philadelphia, PA, USA. POSTER 12. Chirieleison SM , Scelfo, CC, Askew Y, Deasy BM. 2008. Development of Morphological Measurement Scheme Using Open Source Software for Live Cell Imaging Approaches to Stem Cell Biology. International Society for Stem Cell Research, Philadelphia, PA, USA. POSTER 13. Chirieleison SM , Feduska JM, Schugar RC, Sanford SL, Huard J, and Deasy BM. 2008. Identifying Regenerative Populations of Human Muscle-Derived Cells and Tracking Phenotypic Changes in Culture. MidWest Tissue Engineering Consortium. Cincinnati, OH, USA. PODIUM 14. Chirieleison SM , Feduska JM, Schugar RC, Sanford SL, Huard J, Deasy BM. 2008. Identifying Populations of Human Muscle Derived Stem Cells to Participate in Skeletal Muscle Regeneration Based on Phenotypic Differences. Orthopaedic Research Society; San Francisco, CA, USA. POSTER 15. Chirieleison SM* , Young W*, Randall J*, Margulies S. 2007. Computer Controlled Cortical Contusion Device. University of Pennsylvania SEAS Bioengineering Senior Design; Philadelphia, PA, USA. PODIUM *Authors contributed equally 16. Chirieleison SM , Johnson S, Satish L, Post JC, Ehrlich GD, Kathju S. 2006. Differential Expression of Clone 13 in Mammalian Scarless Wound Healing. Pittsburgh Tissue Engineering Initiative Research Symposium; Pittsburgh, PA, USA. PODIUM 17. Sowa GA, Kim JH, Deasy BM, Seo HY, Studer RK, Vo NV, Georgescu HI, Kang JD. 2009. Notochordal cells in intervertebral disc have the ability to differentiate into chondrocyte-like cells in vitro. Orthopaedic Research Society Annual Meeting; Las Vegas, NV, USA. ACKNOWLEDGEMENT 18. Bernieri J, Witt M, Plassmeyer J, Deasy BM, Gharaibeh B, Huard J, Ambrosio F, Wright V. 2008. Muscle Derived Stem Cell Transplantation for Tendon Tissue Engineering: A Methodological Report. Children’s Hospital of Pittsburgh Research Symposium; Pittsburgh, PA, USA. POSTER, ACKNOWLEDGEMENT 19. Plassmeyer J, Wright V, Weiss C, Deasy BM, Huard J, Sowa G, Ambrosio F. 2008. The Effects of Mechanical Strain on Muscle Derived Stem Cell Proliferation. International Society for Stem Cell Research; Philadelphia, PA, USA. ACKNOWLEDGEMENT