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HS Teacher Workshops |
After attending a Department of Biological Sciences workshop, participants are eligible to request science kits containing all the materials, supplies, and equipment required to perform workshop protocols with their students at no cost to the teacher or school district. Five DNA Techniques Kits are available. Kit contents may also be individually tailored for specific needs and goals as determined by discussion between the teacher and the Outreach Coordinator or the DNA Techniques Director upon receipt of the Kit Application Kit Application.
In this protocol, DNA plasmids with ampicillin and kanamycin resistance genes are cut, then religated together in an attempt to form a plasmid containing the resistance genes for both antibiotics. NOTE: Recombination protocol also requires Bacterial Transformation materials. Materials
The transformation protocol may be used along with the recombinant DNA protocol, or as a separate experiment. Bacteria may be transformed with plasmids containing antibiotic resistance genes, or the pGLO plasmid that gives ampicillin resistance along with the green fluorescent protein gene allowing the bacteria to glow in the presence of UV light and arabinose sugar. Materials
DNA Isolation/Restriction Digest/Electrophoresis In this protocol, plasmid DNA is isolated, then digested with restriction enzymes and electrophoresed in order to observe banding patterns. Alternatively, plasmid DNA can be provided for the restriction reaction, or plasmid DNA may be provided as digested and undigested samples that are ready to load onto a gel. Materials for DNA Isolation
Materials for DNA Restriction and Electrophresis
PCR Amplification of D1S80 Locus This protocol may be used as part of a "crime solving" demonstration. The polymerase chain reaction amplifies DNA at the D1S80 locus---a noncoding region on chromosome 1 composed of 16 base pair repeats that can display heterozygous or homozygous phenotypes. PLEASE NOTE: DNA preparation and gel electrophoresis may be performed in the school lab, but the PCR reactions must be done at the Oakland campus due to limited availability of thermocycler required for this protocol. Materials
Extraction of DNA from calf thymus This kit utilizes simple houshold ingredients to extract DNA from calf thymus cells. It allows students to see DNA spooling. Materials
DNA Techniques Kit Application You may request for a kit by completing the following application. After your request is received, we will contact you for more specific information on kit usage. |
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