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   DNA Kit Contents

General Equipment

1 each P-20 Pipetman
1 each P-200 Pipetman
1 each P-1000 Pipetman
1 box Sterile yellow tips
1 box Sterile blue tips
5 each 5 ml disposable pipets
5 each 10 ml disposable pipets
1 each Brinkmann pipet bulbs
1 each Replacement pipet bulb filters
1 jar Sterile 0.5 ml microfuge tubes
1 jar Sterile 1.5 ml microfuge tubes
2 each Sterile 13 X 150 culture tubes
1 each Gibco-BRL Horizon 58 Horizontal Gel Box
1 each 8-well comb
2 each Polaroid Type 667 film
1 each EC-105 Power Supply
1 each Microfuge rack
1 each Microfuge floater
1 each Cell spreader
2 each 50 ml Falcon tubes
1 each 50 ml Falcon tube rack

Solutions & Reagents

1 ml Solution I
1 ml Solution II
1 ml Solution III
1 ml Solution IV
25 ml Solution V
5 ml LB broth
20 ul 0.1 ug/ul lambda DNA
20 ul 2X Restriction Buffer
1 ul EcoRI Restriction Enzyme
1 ul BamHI Restriction Enzyme
1 ul HindIII Restriction Enzyme
500 ul Sterile ddH2O
10 ul 6X Gel Loading Dyes
500 ul 10mg/ml Ethidium Bromide
0.4g HM Agarose
300 ml 1X TBE Buffer
10 ul 0.1 mg/ul pAMP plasmid DNA
10 ul 5X Restriction Buffer with RNase
5 ul BamHI/HindIII Restriction enzyme mixture
10 ul 0.2 mg/ul pKAN plasmid DNA
10 ul 0.2 mg/ul pAMP plasmid DNA
10 ul 2X T4 DNA Ligase Buffer
1 ul T4 DNA Ligase
50 ml CaCl2 Transformation Buffer
100ml Mid-log phase cells
10 ul 0.005 mg/ul pAMP plasmid DNA
10 ul 0.005 mg/ul pKAN plasmid DNA
3 LB-AMP plates
3 LB-KAN plates
3 LB-AMP/KAN plates
3 LB plates

Miscellaneous Supplies

1 each Waste container for used tips
1 each Bunsen burner and striker
250 ml 10% Lysol for clean-up
1 each Permanent marker
1 roll Marking tape
1 each 250 ml Erlenmeyer flask
1 each Ice bucket
250 ml 10% Bleach
200 ml 95% Ethanol for flaming spreaders
1 each Timer

Group Supplies

1 each Clinical centrifuge for transformation exercise
1 each Eppendorf high speed microfuge
1 each Minifuge
1 each 37 C/70 C Water bath
1 each UV transilluminator
1 each Polaroid Hand-Held Camera with small hood
1 box Small latex gloves
1 box Medium latex gloves
1 box Large latex gloves
500 in2 Aluminum foil
1 each Biohazard bag/container for contaminated waste
1 each Hotplate

 
This Site is maintained by the Bioscience Webmaster; this page was last modified 28 May 2009