New ideas arises steadily to optimize and develop tissue engineered scaffolds for culturing cells for various therapeutic application. Different biodegradable polymers particularly Lactides and Glycolides are used as a scaffold with specified porosity in a gradient manner. We are assessing these degradable gradient polymers as an exogenous ECM to study the cellular interaction between degradable and non-degradable matrix. The aim of this project is to design synthetic ECM with degradable gradient property to enhance natural tissue growth and to mimic natural extra cellular matrix.