Histology lab 9: Mar. 29–30, 2005

Female and Male Reproductive Systems

collection slide # label description

Female Reproductive System

Urogen/Repro 3940 Uterus fetus Mammal: l.s. see 7303, 7306

Urogen/Repro 5051 Mammary gland active: sec Ducts and alveoli, smooth muscle, dense connective tissue; milk in lumena.

Urogen/Repro 5522, 5532 Ovary Rabbit sec; Ovary: thin sec: H&E or Ovary mature follicle cat: sec Ovary: cortical region (cortex): 'germinal epithelium' = serosa (mesothelium); tunica albuginea, primordial follicles, thin layer squamous follicle cells; primary follicle (unilaminar or FSH independent): primary oocyte enlarged, layer of cuboidal follicle cells; primary follicle (multilaminar or FSH dependent = growing follicle): primary oocyte surrounded by zona pellucida; stratified layer of follicle cells = zona granulosa surrounded by basement membrane and theca folliculi; secondary follicle: follicular fluid accumulating in separate regions between follicle cells, theca interna, theca externa; tertiary (Graafian) follicle: follicular fluid coalesced in follicular antrum: zona granulosa, cumulus oophorus, corona radiata, theca interna and theca externa.
Also atretic follicle (thickened basement membrane), corpus luteum, corpus albicans
Note: fimbriae and ampulla of uterine tubes in some sections.

Urogen/Repro 5536 Ovary corpus luteum of ovulation: sec or
Ovary corpus luteum of pregnancy: sec Corpus luteum with conspicuous parenchyma of granulosa lutein cells and theca lutein cells (smaller nuclei, denser cytoplasm) associated with connective tissue (also see 3785)

Urogen/Repro 5569 embryo in situ: sec placenta and extraembryonic membranes (you may ignore embryo): chorionic plate, villi, septa, lacunae with maternal blood, vessels with fetal blood, giant cells, cytotrophoblast, syncytial trophoblast, (inverted) yolk sac

Epithel/endo/muscle 7303 Uterus human: follecular phase: sec Thin functional layer (stratum functionalis) of endometrium with relatively straight glands; basal layer (stratum basalis) with sparse glands, straight arteries (superficial) and helical arteries (basal); myometrium.

Epithel/endo/muscle 7306 Uterus human: progravid phase: sec Thick functional layer of endometrium with coiled, active glands; basal layer with active glands, straight arteries (superficial) and helical arteries (basal); myometrium.

Urogen/Repro 7342 Placenta human: sec villi (anchoring and terminal), cytotrophoblast (?), syncytial knot, syncytial trophoblast: fetal red blood cells; intervillus space; decidua basalis, decidual cells.

Urogen/Repro 8679 Mammary Gland Monkey: sec Ducts and alveoli, smooth muscle, dense connective tissue; milk in lumena.

Male Reproductive System

Urogen/Repro 5441 Spermatogenesis rat testis: sec Testis: serosa (tunica vaginalis parietalis and visceral) , fibromyocytes (myoid cells); tunica albuginea; tunica vasculosa; seminiferous tubules, mediastinum testis
Seminiferous tubules: sustentacular or supporting (Sertoli) cells (large, pyramidal nucleus, conspicuous nucleolus): basal compartment: spermatogonia A (slightly flattened, dark and pale nuclei, self-renewing) B (round and lighter nuclei, not self-renewing); adluminal compartment: primary spermatocytes, secondary spermatocytes (difficult to find) spermatids (round and elongating) and spermatozoa: tail piece, head, acrosome; residual bodies
interstitial cells (of Leydig).
straight tubules = tubuli recti (virtually sterile portions at ends of seminiferous tubules;
rete testis (simple cuboidal epithelium) in mediastinum testis;
ductuli efferentes or efferent ductules (simple columnar (ciliated [tall] and nonciliated [short with brush border]) epithelium;
ductus epididymis in some sections.

Urogen/Repro 5453 Testis cat: sec Seminiferous tubules seem to be highly parasitized and consequently abnormal, although you will still find sustentacular cells, spermatogonia, primary spermatocytes, spermatids and some spermatozoa; rete testis and epididymis in some sections.

Urogen/Repro 5454 Mammalian testis: H&E; c.s. & l.s. (see 5441)

Urogen/Repro 5472 Prostate with urethra dog: sec The prostatic urethra is at the (top) edge of section where it can be identified by its transitional epithelium. Remainder of section consists of prostate gland: capsule, ejaculatory ducts, urethral sinuses and urethral crest, septa, lobules,
Prostatic gland: branched tubuloalveolar glands; cuboidal simple to columnar pseudostratified epithelium; fibromuscular stroma; fibroelastic capsule + smooth muscle; septa.
main prostatic glands: peripheral zone (external 2/3rds to 70%: may give rise to prostatic cancer); drains into urethral sinus; prostatic concretions (corpora amylacea, difficult to detect).
transitional or mucosal zone drain into urethral sinuses (may give rise to benign prostatic hyperplasia)
central or submucosal glands: opens directly into urethra

Urogen/Repro 8723 Epididymis primate sec. ductus epididymis: smooth muscle; pseudostratified epithelium (basal and principal cells) with stereocilia; spermatozoa are almost always found in lumen.

Last Updated: Dec. 28, 2004
E-Mail Address: sshostak+@pitt.edu

collection	slide #	label	description
	Female Reproductive System
Urogen/Repro	3940	Uterus fetus Mammal: l.s.	see 7303, 7306
Urogen/Repro	5051	Mammary gland active: sec	Ducts and alveoli, smooth muscle, dense connective tissue; milk in lumena.
Urogen/Repro	5522, 5532	Ovary Rabbit sec; Ovary: thin sec: H&E or Ovary mature follicle cat: sec	Ovary: cortical region (cortex): 'germinal epithelium' = serosa (mesothelium); tunica albuginea, primordial follicles, thin layer squamous follicle cells; primary follicle (unilaminar or FSH independent): primary oocyte enlarged, layer of cuboidal follicle cells; primary follicle (multilaminar or FSH dependent = growing follicle): primary oocyte surrounded by zona pellucida; stratified layer of follicle cells = zona granulosa surrounded by basement membrane and theca folliculi; secondary follicle: follicular fluid accumulating in separate regions between follicle cells, theca interna, theca externa; tertiary (Graafian) follicle: follicular fluid coalesced in follicular antrum: zona granulosa, cumulus oophorus, corona radiata, theca interna and theca externa. Also atretic follicle (thickened basement membrane), corpus luteum, corpus albicans Note: fimbriae and ampulla of uterine tubes in some sections.
Urogen/Repro	5536	Ovary corpus luteum of ovulation: sec or Ovary corpus luteum of pregnancy: sec	Corpus luteum with conspicuous parenchyma of granulosa lutein cells and theca lutein cells (smaller nuclei, denser cytoplasm) associated with connective tissue (also see 3785)
Urogen/Repro	5569	embryo in situ: sec	placenta and extraembryonic membranes (you may ignore embryo): chorionic plate, villi, septa, lacunae with maternal blood, vessels with fetal blood, giant cells, cytotrophoblast, syncytial trophoblast, (inverted) yolk sac
Epithel/endo/muscle	7303	Uterus human: follecular phase: sec	Thin functional layer (stratum functionalis) of endometrium with relatively straight glands; basal layer (stratum basalis) with sparse glands, straight arteries (superficial) and helical arteries (basal); myometrium.
Epithel/endo/muscle	7306	Uterus human: progravid phase: sec	Thick functional layer of endometrium with coiled, active glands; basal layer with active glands, straight arteries (superficial) and helical arteries (basal); myometrium.
Urogen/Repro	7342	Placenta human: sec	villi (anchoring and terminal), cytotrophoblast (?), syncytial knot, syncytial trophoblast: fetal red blood cells; intervillus space; decidua basalis, decidual cells.
Urogen/Repro	8679	Mammary Gland Monkey: sec	Ducts and alveoli, smooth muscle, dense connective tissue; milk in lumena.
	Male Reproductive System
Urogen/Repro	5441	Spermatogenesis rat testis: sec	Testis: serosa (tunica vaginalis parietalis and visceral) , fibromyocytes (myoid cells); tunica albuginea; tunica vasculosa; seminiferous tubules, mediastinum testis Seminiferous tubules: sustentacular or supporting (Sertoli) cells (large, pyramidal nucleus, conspicuous nucleolus): basal compartment: spermatogonia A (slightly flattened, dark and pale nuclei, self-renewing) B (round and lighter nuclei, not self-renewing); adluminal compartment: primary spermatocytes, secondary spermatocytes (difficult to find) spermatids (round and elongating) and spermatozoa: tail piece, head, acrosome; residual bodies interstitial cells (of Leydig). straight tubules = tubuli recti (virtually sterile portions at ends of seminiferous tubules; rete testis (simple cuboidal epithelium) in mediastinum testis; ductuli efferentes or efferent ductules (simple columnar (ciliated [tall] and nonciliated [short with brush border]) epithelium; ductus epididymis in some sections.
Urogen/Repro	5453	Testis cat: sec	Seminiferous tubules seem to be highly parasitized and consequently abnormal, although you will still find sustentacular cells, spermatogonia, primary spermatocytes, spermatids and some spermatozoa; rete testis and epididymis in some sections.
Urogen/Repro	5454	Mammalian testis: H&E; c.s. & l.s.	(see 5441)
Urogen/Repro	5472	Prostate with urethra dog: sec	The prostatic urethra is at the (top) edge of section where it can be identified by its transitional epithelium. Remainder of section consists of prostate gland: capsule, ejaculatory ducts, urethral sinuses and urethral crest, septa, lobules, Prostatic gland: branched tubuloalveolar glands; cuboidal simple to columnar pseudostratified epithelium; fibromuscular stroma; fibroelastic capsule + smooth muscle; septa. main prostatic glands: peripheral zone (external 2/3rds to 70%: may give rise to prostatic cancer); drains into urethral sinus; prostatic concretions (corpora amylacea, difficult to detect). transitional or mucosal zone drain into urethral sinuses (may give rise to benign prostatic hyperplasia) central or submucosal glands: opens directly into urethra
Urogen/Repro	8723	Epididymis primate sec.	ductus epididymis: smooth muscle; pseudostratified epithelium (basal and principal cells) with stereocilia; spermatozoa are almost always found in lumen.