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  Developmental Biology Workshop Kit

After attending a Department of Biological Sciences workshop, participants are eligible to request science kits containing all the materials, supplies, and equipment required to perform workshop protocols with their students at no cost to the teacher or school district.

Four Developmental Biology Kits are available. Kit contents may also be individually tailored for specific needs and goals as determined by discussion between the teacher and Outreach Coordinator upon receipt of the Kit Application.

Sea Urchin Fertilization

This kit can be used to study the process of fertilization and the cascade of physiological events that follow sperm-egg binding. Normal sperm-egg interactions result in the dramatic and easily observable formation of the fertilization envelope, while varying fertilization conditions demonstrates important steps in egg activation. PLEASE NOTE: Maintaning the urchin embryos requires incubation at approximately 19 C (66 F).

Materials:

  • Sea urchins (Type depends on availability)
  • 0.5 M KCl
  • Artificial sea water (with calcium)
  • Calcium-free sea water
  • Artificial sea water, pH 5.0
  • 0.4 mg/ml soybean trypsin inhibitor in ASW
  • Ionomycin (calcium ionophore) in DMSO
  • 25 ml syringe with 18 gauge needle
  • Petri dishes
  • 1.5 ml microcentrifuge tubes
  • Disposable 50 ml conical centrifuge tubes
  • Disposable 15 ml conical centrifuge tubes
  • Disposable Pasteur pipets
  • 12-well culture dishes
  • Microscope slides
  • Coverslips
  • Double-sided tape
  • Compound microscopes
  • Protocols

Sea Urchin Development

One of the most important factors in cell fate determination is cell-cell contact. This protocol demonstrates the alteration of cell fate with the interference of cell signaling pathways due to the introduction of metal ions. PLEASE NOTE: Maintaining the urchin embryos requires incubation at approximately 19 C (66 F).

Materials:

  • Sea urchins (Type depends on availability)
  • 0.5 M KCl
  • Artificial sea water
  • ASW with 10 and 30 mM LiCl
  • ASW with 0.5 and 1 mM NiCl2
  • 25 ml syringe with 18 gauge needle
  • Petri dishes
  • 1.5 ml microcentrifuge tubes
  • Disposable 50 ml conical centrifuge tubes
  • Disposable 15 ml conical centrifuge tubes
  • Disposable Pasteur pipets
  • 6-well culture dishes
  • Microscope slides
  • Coverslips
  • Double-sided tape
  • Compound microscopes
  • Protocols

Zebrafish Development

Zebrafish development is relatively fast and easy to follow using dissecting microscopes. The effect of ethanol and lithium on embryonic development was studied during the workshop, but teachers may also wish to explore the effect of other teratogens with their students. PLEASE NOTE: Zebrafish spawning requires a tightly controlled light cycle.

Materials:

  • Zebrafish
  • Aquarium with heater, filtration system, thermometer, hood
  • Timer for control of light/dark cycle
  • Spawning chamber
  • Embryo water (60 mg/ml Instant Ocean in distilled water)
  • EW with 2.8% ethanol
  • EW with 0.3 M LiCl
  • 6-well culture dishes
  • Dissecting microscopes
  • Protocols

Chicken Heart Development

During the workshop, participants immunostained chick embryos at 24, 48, and 72 hours of development, but teachers may also have students simply observe the embryos under dissecting microscopes without the staining procedure.

Materials:

  • Fertilized chicken eggs
  • Phosphate-buffered saline (PBS)
  • 0.3% hydrogen peroxide in ethanol
  • 100%, 75%, 50%, and 25% ethanol
  • PBS with 0.1% TX-100
  • PBS with 2% BSA
  • Antibody color-developing reagent
  • MF-20 monoclonal anti-myosin antibody
  • Goat anti-mouse IgG, HRP-conjugated
  • Scissors
  • Forceps
  • Whatman Glass filter papers with holes in center
  • Dissecting microscopes
  • Protocols

Kit Application

You may request for a kit by completing the following application. After your request is received, we will contact you for more specific information on kit usage.

Contact Information
Name
Email
Home phone
School name
School address
School phone
School FAX
 
Kit Request
When did you take our workshop?
Which kit do you need?
Anticipated dates of kit usage
(PLEASE NOTE: Kit availability will depend on previously scheduled events.)
Number of classes to use kit
Total number of students to use kit
Briefly describe experiment to be performed
Additional comments

    

If you have any questions, you may contact:

Dr. Alison Slinskey Legg
Outreach Coordinator

Thomas Seiflein
Assistant Outreach Coordinator

 
This Site is maintained by the Bioscience Webmaster; this page was last modified 6 October 2008